Product Info Summary
SKU: | M00154 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD19 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00154
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD19 Rabbit Monoclonal Antibody catalog # M00154. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD19 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00154)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOFO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD19
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00154 is reactive to CD19 in Human
Applications
M00154 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
119 kDa
Calculated molecular weight
61.128kDa
Background of CD19
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of CD19 expression in Ramos cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:100 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD19 (Source: Uniprot.org, NCBI)
Gene Name
CD19
Full Name
B-lymphocyte antigen CD19
Weight
61.128kDa
Alternative Names
B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12 CD19 B4, CVID3 CD19 molecule B-lymphocyte antigen CD19|B-lymphocyte surface antigen B4|T-cell surface antigen Leu-12|differentiation antigen CD19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD19, check out the CD19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD19 Rabbit Monoclonal Antibody (M00154)
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No publications found for M00154
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Recommended Resources
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD19 Rabbit Monoclonal Antibody
Question
My team were happy with the WB result of your anti-CD19 Rabbit Monoclonal antibody. However we have been able to see positive staining in spleen cell membrane using this antibody. Is that expected? Could you tell me where is CD19 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Based on literature, spleen does express CD19. Generally CD19 expresses in cell membrane. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2020-02-27
Question
I see that the anti-CD19 Rabbit Monoclonal antibody M00154 works with IF, what is the protocol used to produce the result images on the product page?
S. Mangal
Verified customer
Asked: 2020-01-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-20
Question
Is a blocking peptide available for product anti-CD19 Rabbit Monoclonal antibody (M00154)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
We do provide the blocking peptide for product anti-CD19 Rabbit Monoclonal antibody (M00154). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-16
Question
We have been able to see staining in human embryonic kidney. Any tips? Is anti-CD19 Rabbit Monoclonal antibody supposed to stain embryonic kidney positively?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
From literature embryonic kidney does express CD19. From Uniprot.org, CD19 is expressed in spleen, tonsil, blood, b-cell, embryonic kidney, among other tissues. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2019-12-13
Question
Do you have a BSA free version of anti-CD19 Rabbit Monoclonal antibody M00154 available?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD19 Rabbit Monoclonal antibody M00154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-13
Question
We are currently using anti-CD19 Rabbit Monoclonal antibody M00154 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The anti-CD19 Rabbit Monoclonal antibody (M00154) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-04
Question
Does M00154 anti-CD19 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
As indicated on the product datasheet, M00154 anti-CD19 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-28
Question
Here is the WB image, lot number and protocol we used for spleen using anti-CD19 Rabbit Monoclonal antibody M00154. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-11
Question
Will anti-CD19 Rabbit Monoclonal antibody M00154 work for IF with spleen?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
According to the expression profile of spleen, CD19 is highly expressed in spleen. So, it is likely that anti-CD19 Rabbit Monoclonal antibody M00154 will work for IF with spleen.
Boster Scientific Support
Answered: 2019-08-26
Question
We are interested in to test anti-CD19 Rabbit Monoclonal antibody M00154 on human spleen for research purposes, then I may be interested in using anti-CD19 Rabbit Monoclonal antibody M00154 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
The products we sell, including anti-CD19 Rabbit Monoclonal antibody M00154, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-CD19 Rabbit Monoclonal antibody M00154. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-20
Question
Can you help my question with product M00154, anti-CD19 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00154 anti-CD19 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-16
Question
Is this M00154 anti-CD19 Rabbit Monoclonal antibody reactive to the isotypes of CD19?
C. Lewis
Verified customer
Asked: 2016-01-26
Answer
The immunogen of M00154 anti-CD19 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD19. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-01-26
Question
I was wanting to use your anti-CD19 Rabbit Monoclonal antibody for IF for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?
N. Parker
Verified customer
Asked: 2014-11-05
Answer
It shows on the product datasheet, M00154 anti-CD19 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-05
Question
We purchased anti-CD19 Rabbit Monoclonal antibody for WB on embryonic kidney in a previous experiment. I am using human, and I plan to use the antibody for IF next. I would like examining embryonic kidney as well as tonsil in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
J. Mitchell
Verified customer
Asked: 2014-11-03
Answer
I viewed the website and datasheets of our anti-CD19 Rabbit Monoclonal antibody and it appears that M00154 has been tested on human in both WB and IF. Thus M00154 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-11-03